Twilight Sparkle is the main protagonist of the My Little Pony G4 series. She is Shining Armor's young sister, Twilight Velvet and Night Light's daughter, and aunt of Flurry Heart
- Tara Strong, Kira Buckland (My Little Pony: Power Ponies game only), Walah Asmah (Arabic), Liu Ru-ping (Taiwanese), Hong Hai-tian (Chinese mainland), Silan Maria Budak Rasch (Danish), Merel Burmeister (Dutch).
Twilight Sparkle appeared as a unicorn from Season 1 to Season 3. In season 3 finale onward, she is an alicorn.