Twinkleshine

Twinkleshine is a female unicorn pony with an ivory coat, curly pink mane and tail, electric blue eyes, and a cutie mark of three sparkly blue stars. She shares her design with Lemon Hearts, and shares her tail style with Daisy and Golden Harvest. She has speaking roles in Friendship is Magic, part 1, A Canterlot Wedding - Part 1, A Canterlot Wedding - Part 2, Amending Fences, and the IDW comics' story The Fall of Sunset Shimmer. Her design resembles that of G1 Moondancer, whose G4 counterpart Moon Dancer she mentions in her first speaking role.

Depiction in the series
Twinkleshine appears next to "Piña Colada" in both versions of the My Little Pony theme song. In the first version, they appear on the left side of the scene with Twinkleshine to the left. In the second version, they are on the train platform with Twinkleshine on the right. They are also briefly next to each other at town hall in Friendship is Magic, part 1 and at the bowling alley in The Cutie Pox.

Twinkleshine appears in over half of the season one episodes as a background pony. Her first appearance is in Friendship is Magic, part 1, where she, Lemon Hearts, and Minuette meet Twilight Sparkle in Canterlot while carrying presents on their backs. She invites Twilight to Moon Dancer's get-together, but Twilight declines on the grounds that she has "a lot of studying to catch up on." Twinkleshine expresses her exasperation behind Twilight's back and chides her for showing more interest in books than friends. She next appears in the surprise party that Pinkie Pie throws for Twilight.

In The Ticket Master, Twinkleshine is one of the ponies crowding around Twilight in front of the library, and again in the alley that Twilight teleports out of. A handful of frames show her starting to run after Twilight, but she is not part of the throng of background ponies in the chase scene that follows. During her two-second participation in the race, her mane style changes to one resembling that of Cherry Berry and Berryshine.

Despite Rainbow Dash stating in Sonic Rainboom that only Pegasus ponies can walk on clouds, Twinkleshine stands on a cloud in both Fluttershy's and Rainbow's flashbacks in The Cutie Mark Chronicles. When this scene is reused in The Cutie Re-Mark - Part 1, Twinkleshine and other wingless ponies are redesigned as Pegasi.

Twinkleshine appears dressed up in Sweet and Elite. In A Canterlot Wedding - Part 1 and A Canterlot Wedding - Part 2, she is one of Queen Chrysalis's original trio of bridesmaids, along with Minuette and Lyra Heartstrings. Contrary to the fake Princess Cadance's disapproval of her tailored wedding dress and train, Twinkleshine says, "I think they're lovely." During This Day Aria, Twinkleshine appears as one of twelve horn-blowing unicorns at the wedding. Later, when the real Princess Cadance and Twilight Sparkle escape the crystal caves, the three brainwashed bridesmaids try to block their way, but the Princess distracts them with her flower bouquet.

Twinkleshine appears in Too Many Pinkie Pies, in the crowd complaining about the Pinkie Pies. She also appears in One Bad Apple, at the movie theater with Minuette and in the crowd during the Summer Harvest Parade. She appears in Magic Duel, in the crowd watching Twilight levitating animals. She also appears in Sleepless in Ponyville, happily trotting through Ponyville while Cherry Berry and Comet Tail are arguing. In Just for Sidekicks, she appears in front of the Mane Six in line at the Crystal Empire train station, talking to "All Aboard". She also appears in Magical Mystery Cure.

Twinkleshine appears in Princess Twilight Sparkle - Part 1, Princess Twilight Sparkle - Part 2, Flight to the Finish, Rainbow Falls, Pinkie Pride, Simple Ways, Filli Vanilli, It Ain't Easy Being Breezies, For Whom the Sweetie Belle Toils, Leap of Faith, Trade Ya!, Inspiration Manifestation, Equestria Games, Twilight's Kingdom - Part 1, and Twilight's Kingdom - Part 2.

Twinkleshine appears in Bloom & Gloom, Tanks for the Memories, Make New Friends but Keep Discord, Slice of Life, and Princess Spike. In Amending Fences, Twinkleshine is referred to by name for the first time in the series and reconnects with old friends Twilight Sparkle and Moon Dancer. In Do Princesses Dream of Magic Sheep?, she appears in Ponyville's shared dream. In Canterlot Boutique, she appears during Rules of Rarity and at Canterlot Carousel's going-out-of-business sale. In Rarity Investigates!, she watches the Wonderbolts' performance alongside Dark Moon. In Scare Master, she and Amethyst Star laugh together during Nightmare Night.

Trivia

 * Twinkleshine is the first ever background pony to have Nicole Oliver as her voice actress.
 * She is the first background pony unicorn to have Andrea Libman as her voice actress.
 * She is the first background pony to have Cathy Weseluck as her voice actress.
 * She is the first background pony to Tabitha St. Germain as her voice actress.
 * She is the only background pony to have three voice actresses in one episode.
 * In Season 2, she appears mostly in the episodes involving Lyra Heartstrings and Minuette, except for Luna Eclipsed.
 * She is the second pony whose cutie mark is a heart between symmetrically-mirrored forte symbols, after Princess Cadance.
 * In Fame and Misfortune, Twinkleshine appears to have an hourglass cutie mark (which is the same as Minuette).